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Image Search Results
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: IGFBP6 was detected by immunostaining in primary NPC tissues (magnification ×200). Left:, IGFBP6 positive staining; Middle: IGFBP6 negative staining; Right: isotype control staining ( A ) IGFBP6 mRNA was measured in five NPC cell lines (CNE2, CNE1, SUNE1, HK1 and HONE1) via RT-PCR, with GAPDH as an internal control ( B ) Data are representative of three separate experiments. Western blotting of whole-cell lysates to detect IGFBP6 ( C ) IGFBP6 levels in CM from NPC cells as measured by ELISA ( D ) Data are representative of two separate experiments. All data represent means ± SD from triplicates.
Article Snippet:
Techniques: Immunostaining, Staining, Negative Staining, Control, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Correlation of IGFBP6 expression with clinical characteristics in patients with NPC
Article Snippet:
Techniques: Expressing
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Multivariate Cox regression analysis for survival prognostic factors in advanced nasopharyngeal carcinoma
Article Snippet:
Techniques: Expressing
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: CNE2 (upper panel) and HK1 (lower panel) cell proliferation as measured by MTS assay ( A ) Data represent means ± SD from six wells. * P < 0.05 compared to controls (IGFBP6 0 ng/ml). In transwell assays (upper panel), exogenous IGFBP6 inhibited CNE2 and HK1 cell invasion compared to controls ( B ) Invasive Index (%) was calculated (lower panel) according to the manufacturer's instructions. Columns, means of triplicate assays; bars, SE. ** P < 0.01 compared to controls.
Article Snippet:
Techniques: MTS Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: CNE2 cells were stably transfected with IGFBP6-shRNA. Real-time RT-PCR confirmed knockdown efficiency ( A ) Bars, ± SE. Data are representative of three separate experiments. Western blotting confirmed IGFBP6 knockdown ( B ) IGFBP6 knockdown induced tumor cell proliferation compared to controls ( C ) Representative wound-healing assay images ( D ) IGFBP6 knockdown increased tumor cell migration ( E ) Data represent means ± SD. * P < 0.05 compared to controls. Western blotting revealed GSK3β/β-catenin/cylin D1 pathway activation as a result of IGFBP6 knockdown ( F ).
Article Snippet:
Techniques: Stable Transfection, Transfection, shRNA, Quantitative RT-PCR, Knockdown, Western Blot, Wound Healing Assay, Migration, Activation Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Silencing IGFBP6 expression in CNE2 cells promotes tumor metastasis in a mouse model
Article Snippet:
Techniques: Expressing
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: IGFBP6 was detected by immunostaining in primary NPC tissues (magnification ×200). Left:, IGFBP6 positive staining; Middle: IGFBP6 negative staining; Right: isotype control staining ( A ) IGFBP6 mRNA was measured in five NPC cell lines (CNE2, CNE1, SUNE1, HK1 and HONE1) via RT-PCR, with GAPDH as an internal control ( B ) Data are representative of three separate experiments. Western blotting of whole-cell lysates to detect IGFBP6 ( C ) IGFBP6 levels in CM from NPC cells as measured by ELISA ( D ) Data are representative of two separate experiments. All data represent means ± SD from triplicates.
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: Immunostaining, Staining, Negative Staining, Control, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Correlation of IGFBP6 expression with clinical characteristics in patients with NPC
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: Expressing
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Multivariate Cox regression analysis for survival prognostic factors in advanced nasopharyngeal carcinoma
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: Expressing
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: CNE2 (upper panel) and HK1 (lower panel) cell proliferation as measured by MTS assay ( A ) Data represent means ± SD from six wells. * P < 0.05 compared to controls (IGFBP6 0 ng/ml). In transwell assays (upper panel), exogenous IGFBP6 inhibited CNE2 and HK1 cell invasion compared to controls ( B ) Invasive Index (%) was calculated (lower panel) according to the manufacturer's instructions. Columns, means of triplicate assays; bars, SE. ** P < 0.01 compared to controls.
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: MTS Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: CNE2 cells were stably transfected with IGFBP6-shRNA. Real-time RT-PCR confirmed knockdown efficiency ( A ) Bars, ± SE. Data are representative of three separate experiments. Western blotting confirmed IGFBP6 knockdown ( B ) IGFBP6 knockdown induced tumor cell proliferation compared to controls ( C ) Representative wound-healing assay images ( D ) IGFBP6 knockdown increased tumor cell migration ( E ) Data represent means ± SD. * P < 0.05 compared to controls. Western blotting revealed GSK3β/β-catenin/cylin D1 pathway activation as a result of IGFBP6 knockdown ( F ).
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: Stable Transfection, Transfection, shRNA, Quantitative RT-PCR, Knockdown, Western Blot, Wound Healing Assay, Migration, Activation Assay
Journal: Oncotarget
Article Title: IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
doi: 10.18632/oncotarget.11886
Figure Lengend Snippet: Silencing IGFBP6 expression in CNE2 cells promotes tumor metastasis in a mouse model
Article Snippet: Recombinant human IGFBP6 (rhIGFBP6) and the
Techniques: Expressing